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人胚胎肺成纖維細(xì)胞WI-38 WI38細(xì)胞
產(chǎn)品時(shí)間：2016-07-21
人胚胎肺成纖維細(xì)胞WI-38 [WI38]細(xì)胞,原代細(xì)胞及傳代細(xì)胞公司專業(yè)培養(yǎng)，想更具體了解細(xì)胞形態(tài)特征、細(xì)胞生長特性、細(xì)胞特種特性、細(xì)胞傳代方法、細(xì)胞傳代情況、細(xì)胞凍存條件情況，請?jiān)儐栁覀児緦I(yè)細(xì)胞銷售人員。
本產(chǎn)品僅用于科研，不用于臨床
細(xì)胞中文名稱人胚胎肺成纖維細(xì)胞
細(xì)胞英文名稱WI-38 [WI38]
物種人
細(xì)胞形態(tài)特征成纖維細(xì)胞樣
細(xì)胞生長特性人胚胎肺成纖維細(xì)胞WI-38 [WI38]細(xì)胞貼壁生長
細(xì)胞特種特性Leonard Hayflick建系；有限傳代細(xì)胞系；壽命為50±10代（倍增時(shí)間24h）；來自妊娠3個(gè)月的正常胚胎肺組織。該細(xì)胞系是*個(gè)用于人疫苗制備的人二倍體細(xì)胞；培養(yǎng)基中添加TNFα可以加快細(xì)胞生長
培養(yǎng)基MEM-EBSS: Minimum Essential Medium （MEM Eagles with Earle's Balanced Salts）
血清10%FBS
細(xì)胞傳代方法1:2~1:4傳代；2~3天換液1次
細(xì)胞傳代情況P19
細(xì)胞凍存條件基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS
支原體檢測陰性
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